Coding

Part:BBa_K2398558:Design

Designed by: Thore Buergel, Lukas Adam, Catharina Gandor, Marita Klein, Jan Mathony, Pauline Pfuderer, Lukas Platz, Moritz Przybilla, Max Schwendemann, Julius Upmeier zu Belzen   Group: iGEM17_Heidelberg   (2017-10-27)


beta-glucuronidase_N566S


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 506
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created from the wildtype E.coli beta-glucuronidase. One or more amino acid substitutions were introduced. It was used for protein expression under a lac inducible T7 promoter.


Source

todo

References